Centrifuge Operation
Centrifuge Operation
The centrifuge is a very common separation instrument in the laboratory. It can be devided in to several series based on different way.
Speed Classification: low-speed centrifuge, high-speed centrifuge, ultracentrifuge, etc.
Temperature Classification:refrigerated centrifuge and normal temperature centrifuge
Capacity Classification: microcentrifuge, large-capacity centrifuge, and ultra-large-capacity centrifuge
Appearance Classification: desktop centrifuges and floor-standing centrifuges
The most important thing to use centrifuge is safe. A runaway centrifugal force can do a lot of damage.
Therefore, we should pay more attention to whether the centrifuge tube is balanced, whether the rotation speed exceeds the setting, whether the rotor is corroded, etc.
Centrifuge operation:
1.When using various centrifuges, the centrifuge tube and its contents must be balanced in advance. The weight difference during balancing cannot exceed the range specified in the instructions of each centrifuge. Different rotors of each centrifuge have their own allowable differences. A single number of tubes must never be loaded in the rotor, and when the rotor is only partially loaded, the tubes must be placed in the rotor symmetrically to each other in order to distribute the load evenly around the rotor.
2. When centrifuging at a temperature lower than room temperature. The rotor should be pre-cooled in a refrigerator or in the rotor chamber of a centrifuge before use.
If there is any abnormal sound, it should be stopped immediately to check and troubleshoot in time.
4.When loading the solution, following the specific operating instructions of the various centrifuges, Choose a suitable centrifuge tube according to the nature and volume of the liquid to be centrifuged. It is strictly forbidden to use centrifuge tubes that are significantly deformed, damaged or aged.
After each use, the rotor must be carefully checked, cleaned and dried in time. The rotor is a part of the centrifuge that needs to be protected.