Centrifuge Operation

View:807 Time:2022-07-14 12:29:51

Centrifuge Operation 
The centrifuge is a very common separation instrument in the laboratory. It can be devided in to several series based on different way.
Speed Classification: low-speed centrifuge, high-speed centrifuge, ultracentrifuge, etc.
Temperature Classification:refrigerated centrifuge and normal temperature centrifuge
Capacity Classification: microcentrifuge, large-capacity centrifuge, and ultra-large-capacity centrifuge
Appearance Classification: desktop centrifuges and floor-standing centrifuges

The most important thing to use centrifuge is safe. A runaway centrifugal force can do a lot of damage.
 Therefore, we should pay more attention to whether the centrifuge tube is balanced, whether the rotation speed exceeds the setting, whether the rotor is corroded, etc.

Centrifuge operation:
1.When using various centrifuges, the centrifuge tube and its contents must be balanced in advance. The weight difference during balancing cannot exceed the range specified in the instructions of each centrifuge. Different rotors of each centrifuge have their own allowable differences. A single number of tubes must never be loaded in the rotor, and when the rotor is only partially loaded, the tubes must be placed in the rotor symmetrically to each other in order to distribute the load evenly around the rotor.

2. When centrifuging at a temperature lower than room temperature. The rotor should be pre-cooled in a refrigerator or in the rotor chamber of a centrifuge before use.


3.Do not leave before centrifuge not reach to the setting speed.  observing the centrifuge are working properly at any time.

If there is any abnormal sound, it should be stopped immediately to check and troubleshoot in time.


4.When loading the solution, following the specific operating instructions of the various centrifuges, Choose a suitable centrifuge tube according to the nature and volume of the liquid to be centrifuged. It is strictly forbidden to use centrifuge tubes that are significantly deformed, damaged or aged.


Some centrifuge tubes have no caps, and the liquid should not be filled too much to prevent the rotor from being thrown out during centrifugation. which cause the rotor to be unbalanced, rusted or corroded. The centrifuge tube for the ultracentrifuge is often required to be filled with liquid to prevent the upper part of the plastic centrifuge tube from being dented and deformed during centrifugation.
After each use, the rotor must be carefully checked, cleaned and dried in time. The rotor is a part of the centrifuge that needs to be protected.  

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